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DNA Analysis: Methodology

•

KRAS

analyses was centrally performed

by Next Generation Sequencing (NGS)

•

Failed samples were rescued by additional

techniques (WT blocking, Taqman, …)

•

Extended

RAS

and

BRAF

analyses were

centrally analyzed by NGS

Cut-off : ≥ 5% VAF

≥ 200 depth

Tejpar, World GI 2017