DNA Analysis: Methodology
•
KRAS
analyses was centrally performed
by Next Generation Sequencing (NGS)
•
Failed samples were rescued by additional
techniques (WT blocking, Taqman, …)
•
Extended
RAS
and
BRAF
analyses were
centrally analyzed by NGS
Cut-off : ≥ 5% VAF
≥ 200 depth
Tejpar, World GI 2017