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DNA Analysis: Methodology

KRAS

analyses was centrally performed

by Next Generation Sequencing (NGS)

Failed samples were rescued by additional

techniques (WT blocking, Taqman, …)

Extended

RAS

and

BRAF

analyses were

centrally analyzed by NGS

Cut-off : ≥ 5% VAF

≥ 200 depth

Tejpar, World GI 2017