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A comprehensive panel required: a Pilot study with Amplicon-seq in mCRC

n=90 (RAS mutant enriched)

NGS VHIO-card (Amplicon-seq), 60 genes

65 samples with available BEAMing test:

25 WT by BEAMing, 21 by NGS (3 likely FP)

40 mutant by BEAMing, 33 by NGS (7 missed by NGS MAF <0.5%)

r

2

0.7