16 / 30
We are around 2,8 – 3,5 SSCS for 1 DCS, maybe due to panel desing intended to capture both strands of DNA.
This would mean an improvement compared to the other two published methods.
Duplex sequencing Kennedy et al., 2014
CAPP-seq Newman et al., 2016
SF3B1
KIT1
ROS
FGFR2
BRCA2
NF1
RNF43
CTNNB1 IDH1
NRAS
ALK1
PDGFRA MET
RET
BRCA1
TP53
AKT1
ESR1
IDH2
PIK3Ca
FGFR3
APC
EGFR ERBB3
MAP2K1 ERBB2
BRAF
FBXW7 KRAS
TertPromoter
VHIO-plasma panel
Best option for a possible routine?
Includes several unstable homopolymers: MSI