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Step 2: biological activity
F(ab’)2-related
Fc-F(ab’)2-related
Fc-related
In Vitro TNFα Neutralization
Suppression of T Cell Proliferation by
Induced Regulatory Macrophages in MLR
Assay
C1q Binding Affinity
TNFα Binding Affinity (ELISA)
Quantitation of Induced Regulatory
Macrophages by FACS Analysis
Binding to Fc Receptors
•
FcγRIIIa (V Type) / FcγRIIIa (F Type),
FcγRIIIb,
•
FcγRIIa, FcγRIIb
•
FcγRI
•
FcRn
Cell Based Binding Affinity
Induced Regulatory Macrophage-
mediated Wound Healing
Ex Vivo Binding to NK Cells
•
1% BSA
•
50% Serum
Apoptosis (FACS) by Reverse
Signaling
CDC
Inhibition of Cytokine Release by
Reverse Signaling
ADCC in NK, PBMCs & WB
•
NK Cells
•
PBMCs
•
Whole Blood
Suppression of Cytokine Secretion
in Caco-2 Cells by Blocking sTNFα
ADCC using LPS-stimulated Monocytes
as Target Cells.
Expression Level of tmTNFα on
Monocyte/Macrophages from IBD
Patients